FETAL LUNG PROFILE AMNIOTIC FLUID

General Information

HLAB/HOL Code: FTLNG
UPHSM LIS Test #: 6924
Schedule: Monday - Sunday
Testing Time: 1 day
Testing Lab: Mayo Labs

Specimen Info
Only 1 specimen type required, unless otherwise specified

Volume: 10 mL
Temperature: Frozen
Tube Type: 1 Amniotic fluid container
Collection Info:
10 mL of amniotic fluid. Centrifuge at 1000 rpm or 200 x g for 10 minutes, separate supernatant, and send both supernatant and sediment
frozen in separate plastic vials on dry ice. Do not send specimens contaminated with blood.
NOTE:
1. Include estimate of duration of pregnancy in weeks on request form.
2. Label specimens appropropriately sediment and supernatant.

Methods

Thin-layer Chromatography (TLC) with Quantitation by Densitometry
Includes lecithin-sphingomyelin ratio (L/S), and phosphatidylglycerol (PG), semi-quantitative on amniotic fluid.

Reference Range

Lecithin

Sphingomyelin Phosphatidyl- Interpretation
Ratio Glycerol

<2.5 Absent Immature
> or =2.5 Absent Indeterminate
<2.5 Trace Indeterminate
> or =2.5 Trace Mature
Any ratio Present Mature

Component Info

Name: L/S RATION (LSRTO)
Method: TLC
CPT Code: 83661
Ref Range:
Comp Units:

Name: PHOSPHATIDYL-GLYCEROL (PTGLY)
Method: TLC
CPT Code: 84081
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Comp Units: