FISH ANALYSIS RB1/13Q34
General Information
HLAB/HOL Code: FISH
UPHSM LIS Test #: 1454
Schedule: Monday-Friday
Testing Time: 1-2 Days
Testing Lab: UP Health System-Marquette
UPHSM LIS Test #: 1454
Schedule: Monday-Friday
Testing Time: 1-2 Days
Testing Lab: UP Health System-Marquette
Specimen Info
Only 1 specimen type required, unless otherwise specified
Volume: 3.0 mL Bone Marrow or 5.0 mL Blood
Temperature: Ambient
Tube Type: Bone Marrow or Malignant Blood
Collection Info:
Bone Marrow is the tissue of choice for initial diagnosis of most neoplastic specimens. Malignant blood can be used in certain cases to monitor minimal residual disease. Prefer a minimum of 3.0 mL of bone marrow or 5.0 mL of malignant blood. Draw into green-top (sodium heparin) tube(s), invert several times to mix (clotted specimens may not work and can compromise results). Label vial with the patient's name and a unique identifying number (MGHS encounter # or MR #). Maximum time from collection should be 24 hours.
SPECIMENS CANNOT BE FROZEN
Temperature: Ambient
Tube Type: Bone Marrow or Malignant Blood
Collection Info:
Bone Marrow is the tissue of choice for initial diagnosis of most neoplastic specimens. Malignant blood can be used in certain cases to monitor minimal residual disease. Prefer a minimum of 3.0 mL of bone marrow or 5.0 mL of malignant blood. Draw into green-top (sodium heparin) tube(s), invert several times to mix (clotted specimens may not work and can compromise results). Label vial with the patient's name and a unique identifying number (MGHS encounter # or MR #). Maximum time from collection should be 24 hours.
SPECIMENS CANNOT BE FROZEN
Methods
Interphase set-up and analysis. A minimum of 200 inerphase nuclei will be analyzed.
Clinical Utilities
Diagnosis of loss of RB1 gene located on chromosome 13 within the q14 band region. Loss of RB1 indicating 13q deletion or monosomy 13 can be found
in many disorders such as multiple myeloma, chronic lymphocytic leukemia, and myelodysplasia.
in many disorders such as multiple myeloma, chronic lymphocytic leukemia, and myelodysplasia.
CPT Codes
88271x2 DNA Probe, Each
88275 - Interphase in situ Hybridization
88291 - Interpretation and Reporting
As needed, at additional charge:
88237- Cell Culture-Lymphocytes
88275 - Interphase in situ Hybridization
88291 - Interpretation and Reporting
As needed, at additional charge:
88237- Cell Culture-Lymphocytes
* The CPT codes provided are based on AMA guidelines and are for informational purposes only. CPT coding
is the sole responsibility of the billing party. Please direct any questions regarding coding to the payer being billed.
Reference Range
An interpretive report will be provided.
