General Information

UPHSM LIS Test #: 1384
Schedule: Monday - Friday
Testing Time: 2 - 5 days
Testing Lab: UP Health System-Marquette

Specimen Info
Only 1 specimen type required, unless otherwise specified

Temperature: Ambient
Tube Type: See Collection Info
Collection Info:
Bone marrow or whole blood:
Collect at least 3.0 mL bone marrow or 5.0 mL whole blood in sodium heparin (green-top) tube(s), invert several times to mix (clotted specimens can compromise results). Maximum time from collection should be 24 hours. Maximum time from collection should be 24 hours.

Fresh Tissue:
Obtain 1 cm(3) of fresh tissue. Place specimen in a screw-capped, sterile container with sterile HBBS, Ringer's solution or normal saline. Send the specimen immediately after resection. Specimen must reach the laboratory within 24 hours; if time is delayed send specimen refrigerated.

Formalin-fixed, paraffin embedded tissue (FFPET):
Specimen must be immediately fixed in 10% neutral buffered formalin; the volume of formalin should be 10 times the volume of the specimen.   Optimum fixation time is 12-24 hours, not to exceed 48 hours or be less than 6 hours. Tissue should then be placed in a paraffin-embedded tissue block. Maintain specimen at room temperature. Label specimen containers with patient's name and a unique identifying number.
FFPET Limitations or Unacceptable Conditions:
Specimen fixed in fixative other than 10% neutral buffered formalin or undergo decalcification.

Please provide:
(1) Pathology Report
(2) H & E of the fixed specimen. Region of invasive carcinoma marked by a pathologist on the H & E.


Interphase analysis of cells for BCL6, MYC, IGH and BCL2 gene rearrangement utilizing breakapart DNA probe strategy.  Detection of gene rearrangement will be reflexed to correlating dual fusion probe strategies, MYC/IGH, CCND1/IGH and BCL2/IGH fusion.  FFPET may be ordered as a panel or a la carte.

Clinical Utilities

Detection of abnormalities involving BCL6, MYC, IGH, BCL2, t(8;14), t(11;14), and t(14;18) most commonly associated with B cell lymphoma.

CPT Codes

Bone Marrow/Whole Blood/Fresh Tissue:
88271 x 8 DNA probe, each
88275 x 3 Interphase in situ Hybridization

As needed CPT codes (additional charge):
88237 Cell Culture-BM/Malignant
88239 Tissue culture for neoplastic disorders; solid tumor
88271 DNA probe, each
88275 Interphase in situ Hybridization

Paraffin Embedded Tissue:
88271 X 8 DNA probe, each
88275 X 2 Interphase in situ Hybridization

* The CPT codes provided are based on AMA guidelines and are for informational purposes only. CPT coding
is the sole responsibility of the billing party. Please direct any questions regarding coding to the payer being billed.

Reference Range

An interpretive report will be provided.