FISH ANALYSIS ALL (ACUTE LYMPHOBLASTIC LEUKEMIA) PANEL

General Information

HLAB/HOL Code: FISH
UPHSM LIS Test #:
Schedule: Monday - Friday
Testing Time: 2 - 5 days
Testing Lab: UP Health System-Marquette

Specimen Info
Only 1 specimen type required, unless otherwise specified

Volume: 3.0mL Bone Marrow or 5.0mL peripheral blood
Temperature: Ambient
Tube Type: 1 sodium heparin (green top)
Collection Info:
Prefer a minimum of 3.0 mL of bone marrow or 5.0 mL of malignant blood. Draw into green-top (sodium heparin) tube(s), invert several times to mix (clotted specimens may not work and can compromise results). Label vial with the patient's name and a unique identifying number. Maximum time from collection should not exceed 24 hours.
SPECIMENS CANNOT BE FROZEN

Methods

Interphase set-up and analysis of 200 cells for chromosome centromeres 4, 10 and 17 and rearrangement of MLL gene; minimum of 500 nuclei for BCR/ABL1 fusion and ETV6/RUNX1 fusion.

Clinical Utilities

Detection of abnormalities involving hyperdiploidy (trisomy of chromosomes 4, 10 & 17), rearrangement of the MLL gene, t(9;22) BCR-ABL1 and t(12;21) ETV6-RUNX1 fusion most commonly associated with acute lymphoblastic leukemia

CPT Codes

88271 x 9 DNA probe, each
88275 x 5 Interphase in situ Hybridization

As needed CPT codes at additional charge
88237 Cell culture Bone Marrow/Malignant Blood.

* The CPT codes provided are based on AMA guidelines and are for informational purposes only. CPT coding
is the sole responsibility of the billing party. Please direct any questions regarding coding to the payer being billed.

Reference Range

An interpretative report will be provided.