FISH ANALYSIS POC PLOIDY DETERMINATION
General Information
HLAB/HOL Code: FISH
UPHSM LIS Test #:
Schedule: Monday - Friday
Testing Time: 2 - 5 days
Testing Lab: UP Health System-Marquette
UPHSM LIS Test #:
Schedule: Monday - Friday
Testing Time: 2 - 5 days
Testing Lab: UP Health System-Marquette
Specimen Info
Only 1 specimen type required, unless otherwise specified
Volume:
Temperature: Ambient
Tube Type: Paraffin-embedded Tissue
Collection Info:
Specimen must be fixed in 10% neutral buffered formalin fixed paraffin embedded tissue block, the volume of formalin should be 10 times the volume of the specimen. Optimum fixation time 12 to 24 hrs but not to exceed 48 hrs or be less than 6 hrs. Maintain the specimen at room temperature. Do not freeze.
Please provide:
1. Pathology Report
2. H & E of the fixed specimen. Region of target tissue marked by a pathologist on the H & E.
Limitations or Unacceptable Conditions:
Specimen fixed in fixative other than 10% neutral buffered formalin or decalcified.
Temperature: Ambient
Tube Type: Paraffin-embedded Tissue
Collection Info:
Specimen must be fixed in 10% neutral buffered formalin fixed paraffin embedded tissue block, the volume of formalin should be 10 times the volume of the specimen. Optimum fixation time 12 to 24 hrs but not to exceed 48 hrs or be less than 6 hrs. Maintain the specimen at room temperature. Do not freeze.
Please provide:
1. Pathology Report
2. H & E of the fixed specimen. Region of target tissue marked by a pathologist on the H & E.
Limitations or Unacceptable Conditions:
Specimen fixed in fixative other than 10% neutral buffered formalin or decalcified.
Methods
Fluorescent in situ hybridization (FISH) pretreatment of 5 µm cut paraffin embedded tissue slide. Processing of tissue and analysis of 60 for each FISH probe site. Probes specific for chromosome centromeres X, Y, 1 and 17 are utilized for the detection of diploid and triploid signal patterns.
Clinical Utilities
Testing is utilized for the distinction of molar and partial molar pregnancies when pathology results are inconclusive.
CPT Codes
88271 x 4, 88274 x 1, 88291 x1
* The CPT codes provided are based on AMA guidelines and are for informational purposes only. CPT coding
is the sole responsibility of the billing party. Please direct any questions regarding coding to the payer being billed.
Reference Range
An interpretative report will be provided.
Negative (not partial mole): diploid signal pattern
Positive (partial mole): triploid signal pattern
Negative (not partial mole): diploid signal pattern
Positive (partial mole): triploid signal pattern
