MYOSITIS ANTIBODY 2 PANEL

General Information

HLAB/HOL Code: MYOAB
UPHSM LIS Test #: 7083
Schedule: Batched weekly
Testing Time: 10-14 days
Testing Lab: RDL Reference Laboratory

Specimen Info
Only 1 specimen type required, unless otherwise specified

Volume: 3.0 mL Serum
Temperature: Refrigerate
Tube Type: 1 Red top tube or serum gel tube
Collection Info:
Spin down and send 3 mL of serum refrigerated in plastic vial.
Note: 1. Indicate serum on request form.
2. Label specimen appropriately (serum).

Volume: 3.0 ml Plasma
Temperature: Refrigerate
Tube Type: 1 LAV EDTA tube
Collection Info:
Spin down and send 3 mL of EDTA plasma refrigerated in plastic vial.
Note: 1. Indicate plasma on request form.
2. Label specimen appropriately (plasma).

Specimen Acceptability

Specimen stability: Refrigerated (preferred): 7 days
Frozen: 60 days

Methods

RIPA and Enzyme Immunoassay (EIA)

Clinical Utilities

1. Anti-Jo 1 Abs are found in subset of myositis patients characterized by interstitial lung disease, systemic polyarthritis, Raynaud’s Phenomena, fever and Mechanic’s Hand (anti-synthetase syndrome).
2. Anti-Jo 1 appears to be a marker for interstitial lung disease in polymyositis.

CPT Codes

83516/Mi-2,
83516/PL12
83516/PL-7
83516/EJ
83516/OJ
83516/SRP
83516/Ku
83516/U2 snRNP
83516/Anti-PM/Scl Ab
86235/Anti-Jo 1 Ab

* The CPT codes provided are based on AMA guidelines and are for informational purposes only. CPT coding
is the sole responsibility of the billing party. Please direct any questions regarding coding to the payer being billed.

Reference Range

Interpretative report will be sent.

Mi-2, PL-12, PL-7, EJ, OJ, SRP, Ku, U2 snRNP
Reference Range:  Negative

Anti-PM/Scl Ab, Anti-Jo-1 Ab
Reference Range: <20

EIA Interpretation:
Negative:                      <20 units
Weak Positive:              20 – 39 units
Moderate Positive:        40 – 80 units
Strong Positive:            >80 units