BETA 2 + CARDIOLIPIN + PHOSPHATIDYL SERINE ANTIBODIES

General Information

HLAB/HOL Code: APLSD
UPHSM LIS Test #: 9034
Schedule:
Testing Time:
Testing Lab: Labcorp

Specimen Info
Only 1 specimen type required, unless otherwise specified

Volume: 2.0 (1.0) mL plasma
Temperature: Frozen
Tube Type: Platelet-poor Plasma Citrated (blue-top) and Serum
Collection Info:
Specimen:  Plasma (platelet poor), frozen
Container:  Blue-top (sodium citrate) tube
Special Instructions:  If the patient's hematocrit exceeds 55%, the volume of citrate in the collection tube must be adjusted. Refer to Coagulation Collection Procedures for directions.

Testing requires Citrate Plasma and Serum

Collection:  Citrated plasma samples should be collected by double centrifugation. Blood should be collected in a blue-top tube containing 3.2% buffered sodium citrate. Evacuated collection tubes must be filled to completion to ensure a proper blood to anticoagulant ratio. The sample should be mixed immediately by gentle inversion at least six times to ensure adequate mixing of the anticoagulant with the blood. A discard tube is not required prior to collection of coagulation samples.  When noncitrate tubes are collected for other tests, collect sterile and nonadditive (red-top) tubes prior to citrate (blue-top) tubes. Any tube containing an alternate anticoagulant should be collected after the blue-top tube. Gel-barrier tubes and serum tubes with clot initiators should also be collected after the citrate tubes. Centrifuge for 10 minutes and carefully remove 2/3 of the plasma using a plastic transfer pipette, being careful not to disturb the cells. Deliver to a plastic transport tube, cap, and re-centrifuge for 10 minutes. Use a second plastic pipette to remove the plasma, staying clear of the platelets at the bottom of the tube. Transfer the plasma into a aliquot tube with screw cap . Freeze immediately and maintain frozen until tested. To avoid delays in turnaround time when requesting multiple tests on frozen samples, please submit separate frozen specimens for each test requested.

Patient Preparation:  Avoid warfarin (Coumadin®) therapy for two weeks and heparin therapy for two days prior to the test. Do not draw from an arm with a heparin lock or heparinized catheter.

Causes for Rejection:  Gross hemolysis; clotted specimen; frozen specimen thawed in transit; tubes <90% full; improper labeling; specimen collected in tube other than 3.2% citrate





Volume: 4.0 (2.0) mL Serum
Temperature: Refrigerate
Tube Type: Red-Top or Gel-Barrier Tube
Collection Info:
Testing requires Citrated Plasma and Serum

Specimen Acceptability

Test requires both Citrated Plasma and Serum.

Methods

Anticardiolipin antibodies:
Enzyme-linked immunosorbent assay (ELISA) detecting isotype-specific ACA binding to a microtiter plate coated with purified cardiolipin antigen

DRVVT:  
Low phospholipid reagent consisting of Russell viper venom with excess calcium is mixed with patient plasma. The time to clot formation is measured photo-optically.

Antiphosphatidylserine Antibodies:
Enzyme-linked immunosorbent assay (ELISA)

Beta 2 glycoprotein 1 antibodies:
Enzyme-linked immunosorbent assay (ELISA) to detect antibodies binding to a microtiter plate coated with purified β2-glycoprotein 1 antigen









CPT Codes

85613, 86146x3, 86147x3, 86148x3

* The CPT codes provided are based on AMA guidelines and are for informational purposes only. CPT coding
is the sole responsibility of the billing party. Please direct any questions regarding coding to the payer being billed.

Reference Range

See Report for Reference Ranges