ASPERGILLUS (GALACTOMANNAN) ANTIGEN

General Information

HLAB/HOL Code: ASPAG
UPHSM LIS Test #: 4254
Schedule:
Testing Time:
Testing Lab: Labcorp

Specimen Info
Only 1 specimen type required, unless otherwise specified

Volume: 2.0 mL (0.5 mL min.) Serum
Temperature: Refrigerate
Tube Type: Plain Red-Top or Gel-Barrier Tube
Collection Info:
Collect specimen using aseptic technique. Avoid opening the specimen after collection. Do not aliquot (Package insert (PI) specifies transport in sealed tubes).
Refrigerate unopened at 2°C to 8°C for five days. After opening, store at 2°C to 8°C for 48 hours. Freeze at: -70°C after five days.

Volume: 2.0 mL (0.50 mL min.) Bronchoalveolar Lavage
Temperature: Refrigerate
Tube Type: Sterile screw-cap, leak-proof container
Collection Info:
Collect specimen using aseptic technique. Avoid opening the specimen after collection. Do not aliquot (Package insert (PI) specifies transport in sealed tubes).
Refrigerate unopened at 2°C to 8°C for five days. After opening, store at 2°C to 8°C for 48 hours. Freeze at: -70°C after five days.

Specimen Acceptability

Stability: Not stable at room temperature.
Stable refrigerated when unopened for five days.
Stable refrigerated opened for 48 hours.
Stable frozen for 14 days.

Methods

EIA

Clinical Utilities

Use:
Patients diagnosed with chronic granulomatous disease and/or Job's syndrome may yield a reduced detection of galactomannan. Reduced assay sensitivity may occur in patients receiving concomitant antifungal therapy. Penicillium species, Alternaria species, Paecilomyces species, Geotrichum species, and Histoplasma species have demonstrated reactivity with the monoclonal antibodies used in the assay and may, therefore, yield a positive test result. Positive results in patients with no clinical signs of disease have been reported, especially in young children.  Most of these are considered to be false positives. Young children may have a positive assay result due to the presence of galactofuranaase contained in various foods (cereals) and milk. Patients receiving pipercillin/tazobactam or semisynthetic beta-lactamase therapy may have false-positive results. False-positive results may also occur with use of PLASMA-LYTE™ for either intravenous hydration or BAL specimen collection.

Limitations:
A negative result does not exclude the possibility of invasive aspergillosis. Patients at risk for invasive aspergillosis should be tested twice weekly. Results close to the cutoff (ie, index of 0.5) should be interpreted with caution and supported by clinical, radiological, or laboratory culture results. BAL samples with an index value between 0.5 and 1.0 have a lower predictive value than those with a value >1.0.

CPT Codes

87305

* The CPT codes provided are based on AMA guidelines and are for informational purposes only. CPT coding
is the sole responsibility of the billing party. Please direct any questions regarding coding to the payer being billed.

Reference Range

Negative