QuantiFERON-TB Gold Plus
General Information
HLAB/HOL Code: QFTTB
UPHSM LIS Test #: 023524
Schedule:
Testing Time: 2-5 Days
Testing Lab: LabCorp
UPHSM LIS Test #: 023524
Schedule:
Testing Time: 2-5 Days
Testing Lab: LabCorp
Specimen Info
Only 1 specimen type required, unless otherwise specified
Volume: 1mL blood in each of the 4 tubes
Temperature: Room Temperature until incubation
Tube Type: QuantiFERON®-TB Gold Plus collection kit
Collection Info:
IF INCUBATED AND SPUN BEFORE SENDING TO UPHS-MARQUETTE PLEASE MARK THAT ON THE BOX PROVIDED WITH THE COLLECTION KIT AND RETURN THE BOX WITH THE TUBES. ALWAYS RETURN SPECIMENS IN THE PROVIDED BOX.
Collection: Blood collection tubes should be at room temperature at the time of collection. Refer to collection instructions included with draw kit. Special specimen collection kit contains four gel-barrier tubes as noted above. All four tubes are required for a single test result. Each tube is designed to draw only 1 mL and fill time may be longer than other blood collection tubes. Because of the limited vacuum in these tubes, use a needle and holder (not a butterfly) to collect QuantiFERON® specimens. If a butterfly is required, first collect other required tubes or use another Vacutainer® tube to purge the butterfly line of air and then proceed with drawing the QuantiFERON tubes. Fill tubes to the black fill line on the tube. If tubes are underfilled or overfilled (see kit insert), immediately collect a replacement tube. Following proper fill, label the tubes appropriately and shake tubes 10 times firmly enough to ensure the entire surface of the tube is coated with blood cells to solubilize the antigen on the tube walls. After shaking, the volume may fall below the fill line. Do not centrifuge or refrigerate specimens. Return each of the four properly filled, labeled, and shaken tubes to the box labeled "QFT kit." Seal the top by removing tape from the adhesive.
This test is TIME-SENSITIVE. Specimens must be incubated at 37 degrees C for 16 to 24 hours before shipping to LabCorp.
Incubate all 4 QFT-Plus tubes upright at 37°C ± 1°C for 16 to 24 hours. Following incubation centrifuge for 15 minutes at 2,000–3,000 RCF (g)
Temperature: Room Temperature until incubation
Tube Type: QuantiFERON®-TB Gold Plus collection kit
Collection Info:
IF INCUBATED AND SPUN BEFORE SENDING TO UPHS-MARQUETTE PLEASE MARK THAT ON THE BOX PROVIDED WITH THE COLLECTION KIT AND RETURN THE BOX WITH THE TUBES. ALWAYS RETURN SPECIMENS IN THE PROVIDED BOX.
Collection: Blood collection tubes should be at room temperature at the time of collection. Refer to collection instructions included with draw kit. Special specimen collection kit contains four gel-barrier tubes as noted above. All four tubes are required for a single test result. Each tube is designed to draw only 1 mL and fill time may be longer than other blood collection tubes. Because of the limited vacuum in these tubes, use a needle and holder (not a butterfly) to collect QuantiFERON® specimens. If a butterfly is required, first collect other required tubes or use another Vacutainer® tube to purge the butterfly line of air and then proceed with drawing the QuantiFERON tubes. Fill tubes to the black fill line on the tube. If tubes are underfilled or overfilled (see kit insert), immediately collect a replacement tube. Following proper fill, label the tubes appropriately and shake tubes 10 times firmly enough to ensure the entire surface of the tube is coated with blood cells to solubilize the antigen on the tube walls. After shaking, the volume may fall below the fill line. Do not centrifuge or refrigerate specimens. Return each of the four properly filled, labeled, and shaken tubes to the box labeled "QFT kit." Seal the top by removing tape from the adhesive.
This test is TIME-SENSITIVE. Specimens must be incubated at 37 degrees C for 16 to 24 hours before shipping to LabCorp.
Incubate all 4 QFT-Plus tubes upright at 37°C ± 1°C for 16 to 24 hours. Following incubation centrifuge for 15 minutes at 2,000–3,000 RCF (g)
Specimen Acceptability
** Under- or over-filling of the tubes outside of the black box on the side of the tubes may lead to erroneous results. Specimens with volumes outside of the black mark will be rejected. **
Methods
Whole blood interferon-gamma test measuring responses to CD4+ and CD8+ T cell antigens
Clinical Utilities
Indirect test for Mycobacterium tuberculosis infection, to be used in conjunction with risk assessment, radiography, and other medical and diagnostic evaluations.
* This test is not recommended for use for diagnosis of active tuberculosis (TB) infection. It can be used as an aid to detect LATENT TB infection. *
* This test is not recommended for use for diagnosis of active tuberculosis (TB) infection. It can be used as an aid to detect LATENT TB infection. *
CPT Codes
86480
* The CPT codes provided are based on AMA guidelines and are for informational purposes only. CPT coding
is the sole responsibility of the billing party. Please direct any questions regarding coding to the payer being billed.
Reference Range
Negative
