HIV-1 GENOTYPIC PR-RT RESISTANCE

General Information

HLAB/HOL Code: HIVDR
UPHSM LIS Test #: 028430
Schedule:
Testing Time: 2-10 Days
Testing Lab: Mayo

Specimen Info
Only 1 specimen type required, unless otherwise specified

Volume: 2.2 mL
Temperature: Frozen
Tube Type: Plasma (EDTA)
Collection Info:
Collection Instructions:
1. Centrifuge blood collection tube and aliquot plasma into plastic vial per collection tube manufacturer's instructions (eg, centrifuge and aliquot within 2 hours of collection for BD Vacutainer tubes).
2. Freeze aliquoted plasma for shipment.
Additional Information: Specimens submitted for HIV-1 genotyping must contain 1000 copies/mL or more of HIV-1 RNA.

Specimen Acceptability

REJECT DUE TO:
Gross hemolysis: OK
Gross lipemia: OK
Gross icterus: OK

Methods

Reverse Transcription Polymerase Chain Reaction (RT-PCR) followed by Targeted Next-Generation Sequencing (NGS)

Clinical Utilities

Identifying HIV-1 genotypic mutations associated with resistance to nucleotide and non-nucleoside reverse-transcriptase inhibitors, protease inhibitors, and integrase strain transfer inhibitors
Guiding initiation or change of combination antiretroviral therapy in individuals, including children, with HIV-1 infection

CPT Codes

0219U

* The CPT codes provided are based on AMA guidelines and are for informational purposes only. CPT coding
is the sole responsibility of the billing party. Please direct any questions regarding coding to the payer being billed.