CUTANEOUS IMMUNOFLUORESCENCE BIOPSY
General Information
HLAB/HOL Code: CUTBX
UPHSM LIS Test #: 8394
Schedule: Monday - Friday
Testing Time: 2 days
Testing Lab: Mayo Labs
UPHSM LIS Test #: 8394
Schedule: Monday - Friday
Testing Time: 2 days
Testing Lab: Mayo Labs
Specimen Info
Only 1 specimen type required, unless otherwise specified
Volume:
Temperature: Media-AmbRefr OKNo media-Frozen
Tube Type: See Below
Collection Info:
Punch biopsy (4 mm) of uninvolved or involved skin as per complete instructions. Skin or mucosal specimens can be sent by using
either the transport medium or the snap-frozen procedure. The practical value of using transport medium (supplied by Mayo Medical
Laboratories) is recognized for direct immunofluorescence testing. However, we have found a loss in sensitivity of approximately 10% with
the transport medium as compared with snap-frozen tissue. This may necessitate a repeat biopsy if the result seems to be false-negative.
The assay cannot be performed on specimens fixed in formalin.
TRANSPORT MEDIUM METHOD
Immediately drop specimen into provided vial of transport medium. Seal tightly.
SNAP-FROZEN METHOD
1. Immediately drop specimen into liquid nitrogen and allow to freeze thoroughly (do not allow specimen to desiccate). If liquid nitrogen
is not available, the specimen may be frozen by placing it on a small square of aluminum foil on a block of dry ice. The former
is preferred.
2. Immediately wrap specimen carefully in aluminum foil. At no time should the specimen be allowed to thaw. Wrap as you would a party
favor or a piece of taffy candy.
3. Place the wrapped specimen into the prelabeled plastic vial and seal tightly.
4. Send specimen frozen on dry ice.
NOTE:
1. BIOPSY SITE, WHETHER THE BIOPSY WAS OBTAINED FROM SUN-EXPOSED VERSUS UNEXPOSED SKIN, WHETHER IT IS FROM PERILESIONAL,
INVOLVED, OR UNINVOLVED SKIN, AND DATE OF COLLECTION ARE REQUIRED ON REQUEST FORM FOR PROCESSING.
2. Please complete a "Pathology/Dermatology/Cytology Request Form"and forward it with the specimen. This form is supplied
by Mayo Medical Laboratories. Requisitions for this procedure cannot be processed unless the information requested is supplied.
Temperature: Media-AmbRefr OKNo media-Frozen
Tube Type: See Below
Collection Info:
Punch biopsy (4 mm) of uninvolved or involved skin as per complete instructions. Skin or mucosal specimens can be sent by using
either the transport medium or the snap-frozen procedure. The practical value of using transport medium (supplied by Mayo Medical
Laboratories) is recognized for direct immunofluorescence testing. However, we have found a loss in sensitivity of approximately 10% with
the transport medium as compared with snap-frozen tissue. This may necessitate a repeat biopsy if the result seems to be false-negative.
The assay cannot be performed on specimens fixed in formalin.
TRANSPORT MEDIUM METHOD
Immediately drop specimen into provided vial of transport medium. Seal tightly.
SNAP-FROZEN METHOD
1. Immediately drop specimen into liquid nitrogen and allow to freeze thoroughly (do not allow specimen to desiccate). If liquid nitrogen
is not available, the specimen may be frozen by placing it on a small square of aluminum foil on a block of dry ice. The former
is preferred.
2. Immediately wrap specimen carefully in aluminum foil. At no time should the specimen be allowed to thaw. Wrap as you would a party
favor or a piece of taffy candy.
3. Place the wrapped specimen into the prelabeled plastic vial and seal tightly.
4. Send specimen frozen on dry ice.
NOTE:
1. BIOPSY SITE, WHETHER THE BIOPSY WAS OBTAINED FROM SUN-EXPOSED VERSUS UNEXPOSED SKIN, WHETHER IT IS FROM PERILESIONAL,
INVOLVED, OR UNINVOLVED SKIN, AND DATE OF COLLECTION ARE REQUIRED ON REQUEST FORM FOR PROCESSING.
2. Please complete a "Pathology/Dermatology/Cytology Request Form"and forward it with the specimen. This form is supplied
by Mayo Medical Laboratories. Requisitions for this procedure cannot be processed unless the information requested is supplied.
Methods
Direct Immunofluroescence Staining of Cryostat Prepared Skin biopsy Sections for IgG, IgM, IgA, C3, and Fibrinogen Deposition
CPT Codes
88323/cutaneous immunofluorescence, biopsy
88346/x5 direct immunofluorescence staining
88346/x5 direct immunofluorescence staining
* The CPT codes provided are based on AMA guidelines and are for informational purposes only. CPT coding
is the sole responsibility of the billing party. Please direct any questions regarding coding to the payer being billed.
Reference Range
Report includes description and interpretation of staining patterns.
