CHROMOSOME ANALYSIS AMNIOTIC FLUID

General Information

HLAB/HOL Code: CHRAF
UPHSM LIS Test #: 0000
Schedule:
Testing Time: 10-11 Days
Testing Lab: Mayo Labs

Specimen Info
Only 1 specimen type required, unless otherwise specified

Volume: 20-25 mL Amniotic fluid
Temperature: Refrigerated
Tube Type: Sterile Container
Collection Info:
Provide a reason for referral and gestational age with each specimen, and verify the specimen source. The laboratory will not reject testing if this information is not provided, but appropriate testing and interpretation may be compromised or delayed.  
Collection Instructions:
1. Optimal timing for specimen collection is during 14 to 18 weeks of gestation,  but specimens collected at other weeks of gestation are  
     also accepted.
2. Discard the first 2 mL of amniotic fluid.
Additional Information:
1. Place the tube in a refrigerate/ambient mailer (Supply T329).  Fill remaining space with packing material.
2. Unavoidably, about 1% to 2% of mailed in specimens are not viable.
3. Bloody specimens are undesirable.
4. If the specimen does not grow in culture, you will be notified within  7 days of receipt.
Volume: Sterile Fetal body fluid
Temperature: Refrigerated
Tube Type: Sterile Container
Collection Info:
Include entire specimen.
Additional Information:
1. Place the tube in a Refrigerate/Ambient mailer (Supply T329).
2. Fill remaining space with packing material.
3. If the specimen does not grow in culture, you will be notified within  7 days of receipt.
4. Clearly indicate on tube and paperwork that specimen is fetal body fluid.

Methods

Cell Culture Followed by Chromosome Analysis

Clinical Utilities

Generally includes 2 banded karyograms and the analysis of metaphases from 15 colonies. If no metaphase cells are available for analysis, no analysis charges will be incurred.

CPT Codes

88291-Interpretation and report
The following CPT codes will be applied as appropriate:
88235-Tissue culture for amniotic fluid or chorionic villus cells
88269 w/modifier 52- Chromosome analysis, in situ for amniotic fluid cells, <6 colonies, 1 karyotype with banding
88269-Chromosome analysis, in situ for amniotic fluid cells, 6 or greater colonies, 1 karyotype with banding
88267, 88285- Chromosome analysis, amniotic fluid or chorionic villus, greater than 15 cells, 1 karyotype with banding
88267 w/modifier 52- Chromosome analysis, amniotic fluid or chorionic villus, <15 cells, 1 karyotype with banding
88267-Chromosome analysis, amniotic fluid or chorionic villus, 15 cells, 1 karyotype with banding
88280- Chromosome analysis; additional karyotypes, each study
88283-Additional specialized banding technique

* The CPT codes provided are based on AMA guidelines and are for informational purposes only. CPT coding
is the sole responsibility of the billing party. Please direct any questions regarding coding to the payer being billed.

Reference Range

An interpretative report will be provided.