PRENATAL ANEUPLOIDY DETECTION FISH
General Information
HLAB/HOL Code: PADF
UPHSM LIS Test #: 0000
Schedule: Monday - Sunday
Testing Time: 3 days
Testing Lab: Mayo Labs
UPHSM LIS Test #: 0000
Schedule: Monday - Sunday
Testing Time: 3 days
Testing Lab: Mayo Labs
Specimen Info
Only 1 specimen type required, unless otherwise specified
Volume: 20 - 25 mL Amniotic fluid
Temperature: Refrigerated
Tube Type: 1 Amniotic fluid container
Collection Info:
1. Optimal timing for specimen collection is during 14 to 18 weeks of gestation, but specimens collected at other weeks of gestation
are also accepted. Provide gestational age at the time of amniocentesis.
2. Discard the first 2 mL of amniotic fluid.
Additional Information:
1. Place the tubes in a Styrofoam container.
2. Fill remaining space with packing material.
3. Unavoidably, about 1% to 2% of mailed in specimens are not viable.
4. Bloody specimens are undesirable.
5. If the specimen does not grow in culture, you will be notified within 7 days of receipt.
Temperature: Refrigerated
Tube Type: 1 Amniotic fluid container
Collection Info:
1. Optimal timing for specimen collection is during 14 to 18 weeks of gestation, but specimens collected at other weeks of gestation
are also accepted. Provide gestational age at the time of amniocentesis.
2. Discard the first 2 mL of amniotic fluid.
Additional Information:
1. Place the tubes in a Styrofoam container.
2. Fill remaining space with packing material.
3. Unavoidably, about 1% to 2% of mailed in specimens are not viable.
4. Bloody specimens are undesirable.
5. If the specimen does not grow in culture, you will be notified within 7 days of receipt.
Volume: 20 - 30 mg Chorionic villi
Temperature: Refrigerated
Tube Type: 15 mL containing transport media
Collection Info:
1. Collect specimen by the transabdominal or transcervical method.
2. Transfer chorionic villi to a Petri dish containing transport medium.
3. Using a stereomicroscope and sterile forceps, assess the quality and quantity of the villi and remove any blood clots and maternal
decidua.
Temperature: Refrigerated
Tube Type: 15 mL containing transport media
Collection Info:
1. Collect specimen by the transabdominal or transcervical method.
2. Transfer chorionic villi to a Petri dish containing transport medium.
3. Using a stereomicroscope and sterile forceps, assess the quality and quantity of the villi and remove any blood clots and maternal
decidua.
Methods
Fluorescence In Situ Hybridization (FISH)
CPT Codes
88291-Interpretation and report
The following CPT codes will be applied as appropriate based on the number of probe sets applied, culture performed, or cells analyzed as indicated in the test report:
88271x2-DNA probe, each; first probe set
88271x2-DNA probe, each; each additional probe set
88271x3-DNA probe, each; coverage for sets containing 5 probes
88271x2-DNA probe, each; coverage for sets containing 4 probes
88271x1-DNA probe, each; coverage for sets containing 3 probes
88274 w/mod 52-Interphase in situ hybridization, <25 cells, each probe set
88274-Interphase in situ hybridization, 25 to 99 cells, each probe set
88275-Interphase in situ hybridization, 100 to 300 cells, each probe set
The following CPT codes will be applied as appropriate based on the number of probe sets applied, culture performed, or cells analyzed as indicated in the test report:
88271x2-DNA probe, each; first probe set
88271x2-DNA probe, each; each additional probe set
88271x3-DNA probe, each; coverage for sets containing 5 probes
88271x2-DNA probe, each; coverage for sets containing 4 probes
88271x1-DNA probe, each; coverage for sets containing 3 probes
88274 w/mod 52-Interphase in situ hybridization, <25 cells, each probe set
88274-Interphase in situ hybridization, 25 to 99 cells, each probe set
88275-Interphase in situ hybridization, 100 to 300 cells, each probe set
* The CPT codes provided are based on AMA guidelines and are for informational purposes only. CPT coding
is the sole responsibility of the billing party. Please direct any questions regarding coding to the payer being billed.
Reference Range
An interpretative report is provided.
