HERPES SIMPLEX VIRUS (HSV) TYPE 1 AND TYPE 2 SPECIFIC ANTIBODIES IgG
General Information
HLAB/HOL Code: HSVABS
UPHSM LIS Test #: 026577
Schedule:
Testing Time:
Testing Lab: UP Health System-Marquette
UPHSM LIS Test #: 026577
Schedule:
Testing Time:
Testing Lab: UP Health System-Marquette
Specimen Info
Only 1 specimen type required, unless otherwise specified
Volume: 1.0 mL
Temperature: Refrigerate
Tube Type: Serum or Plasma
Collection Info:
Serum and EDTA or Heparinized plasma are the recommended samples.
Temperature: Refrigerate
Tube Type: Serum or Plasma
Collection Info:
Serum and EDTA or Heparinized plasma are the recommended samples.
Specimen Acceptability
Avoid hemolysis
Methods
Multiplex Flow Immunoassay (MFI)
Clinical Utilities
• The BioPlex 2200 HSV-1 & HSV-2 IgG is not intended to be diagnostic for infectious disease and should only be interpreted in the context of other laboratory findings and the total clinical presentation of the patient.
• Samples collected too early in the course of the infection may not have detectable levels of HSV IgG. Negative HSV-2 results may be due to delayed seroconversion.
• Timing of specimen collection for paired sera may be critical. In some patients, antibody titers may rise to significant levels and fall again to lower or undetectable levels with a month. Other patients may not develop significant antibody levels.
• Possible cross reactivity with E.coli, the recombinant vector for the gG1 antigen, has not been evaluated by itself.
• These tests cannot differentiate between recent and old infections, negative HSV-2 antibody results could be due to recent infections attributed to the slow time to seroconversion of the gG-2 response. A positive result does not rule out primary or no-primary episodes.
• HSV serology cannot distinguish genital from no-genital infections.
• Immune complexes or other immunoglobulin aggregates present in patient samples may cause increased non-specific binding and produce false positive results. However, the BioPlex 2200 HSV-1 & HSV-2 IgG kit incorporates a Reagent Blank Bead (RBB) with each test that verifies the absence of significant non-specific binding in serum.
• The predictive value of positive or negative results depends on the population's prevalence and the pretest likelihood of HSV-1 and HSV-2.
• The numerical value of the final result above the cutoff is not indicative of the amount of anti-HSV-1 and 2 IgG antibody present.
• Samples collected too early in the course of the infection may not have detectable levels of HSV IgG. Negative HSV-2 results may be due to delayed seroconversion.
• Timing of specimen collection for paired sera may be critical. In some patients, antibody titers may rise to significant levels and fall again to lower or undetectable levels with a month. Other patients may not develop significant antibody levels.
• Possible cross reactivity with E.coli, the recombinant vector for the gG1 antigen, has not been evaluated by itself.
• These tests cannot differentiate between recent and old infections, negative HSV-2 antibody results could be due to recent infections attributed to the slow time to seroconversion of the gG-2 response. A positive result does not rule out primary or no-primary episodes.
• HSV serology cannot distinguish genital from no-genital infections.
• Immune complexes or other immunoglobulin aggregates present in patient samples may cause increased non-specific binding and produce false positive results. However, the BioPlex 2200 HSV-1 & HSV-2 IgG kit incorporates a Reagent Blank Bead (RBB) with each test that verifies the absence of significant non-specific binding in serum.
• The predictive value of positive or negative results depends on the population's prevalence and the pretest likelihood of HSV-1 and HSV-2.
• The numerical value of the final result above the cutoff is not indicative of the amount of anti-HSV-1 and 2 IgG antibody present.
CPT Codes
86695-Herpes simplex, type 1
86696-Herpes simplex, type 2
86696-Herpes simplex, type 2
* The CPT codes provided are based on AMA guidelines and are for informational purposes only. CPT coding
is the sole responsibility of the billing party. Please direct any questions regarding coding to the payer being billed.
Reference Range
Negative (reported as positive, negative, or equivocal)
