Mycoplasma pneumoniae PCR CSF
General Information
HLAB/HOL Code: MPRP
UPHSM LIS Test #: 026584
Schedule:
Testing Time: 3-4 Days
Testing Lab: Mayo
UPHSM LIS Test #: 026584
Schedule:
Testing Time: 3-4 Days
Testing Lab: Mayo
Specimen Info
Only 1 specimen type required, unless otherwise specified
Volume: Varies
Temperature: Refrigerated
Tube Type: Varies
Collection Info:
SPECIMEN REQUIRED
The high sensitivity of amplification by polymerase chain reaction requires the specimen to be processed in an environment in which contamination of the specimen by Mycoplasmoides pneumoniae DNA is unlikely.
Submit only 1 of the following specimens:
Specimen Type: Respiratory
Sources: Bronchial washing, bronchoalveolar lavage, tracheal secretions, sputum
Container/Tube:
Preferred: Sterile container
Acceptable: Specimen in M4, M4-RT, M5, M6, or universal transport medium
Specimen Volume: 1 mL
Specimen Type: Swab
Sources: Throat, nasal, or nasopharyngeal
Container/Tube:
Preferred: Culture swab transport system (Dacron or rayon swab with aluminum or plastic shaft with either Stuart or Amies liquid medium)
Acceptable: Culture transport swab (Stuart's media) or place swab in M4, M4-RT, M5, M6, universal transport media, or ESwab
Specimen Volume: Swab
Collection Instructions:
1. Collect specimen by swabbing back and forth over mucosa surface to maximize recovery of cells.
2. Place swab back into swab cylinder.
Specimen Type: Fluid
Sources: Pleural, pericardial, cerebrospinal
Container/Tube: Sterile vial
Specimen Volume: 0.5 mL
Temperature: Refrigerated
Tube Type: Varies
Collection Info:
SPECIMEN REQUIRED
The high sensitivity of amplification by polymerase chain reaction requires the specimen to be processed in an environment in which contamination of the specimen by Mycoplasmoides pneumoniae DNA is unlikely.
Submit only 1 of the following specimens:
Specimen Type: Respiratory
Sources: Bronchial washing, bronchoalveolar lavage, tracheal secretions, sputum
Container/Tube:
Preferred: Sterile container
Acceptable: Specimen in M4, M4-RT, M5, M6, or universal transport medium
Specimen Volume: 1 mL
Specimen Type: Swab
Sources: Throat, nasal, or nasopharyngeal
Container/Tube:
Preferred: Culture swab transport system (Dacron or rayon swab with aluminum or plastic shaft with either Stuart or Amies liquid medium)
Acceptable: Culture transport swab (Stuart's media) or place swab in M4, M4-RT, M5, M6, universal transport media, or ESwab
Specimen Volume: Swab
Collection Instructions:
1. Collect specimen by swabbing back and forth over mucosa surface to maximize recovery of cells.
2. Place swab back into swab cylinder.
Specimen Type: Fluid
Sources: Pleural, pericardial, cerebrospinal
Container/Tube: Sterile vial
Specimen Volume: 0.5 mL
Specimen Acceptability
REJECT DUE TO:
Cotton or calcium alginate-tipped swab, wooden shaft swab, transport swab
containing gel or charcoal
Port-a-Cul tube
Anaerobic fluid vials
Dry swab (no pledget or sponge)
Cotton or calcium alginate-tipped swab, wooden shaft swab, transport swab
containing gel or charcoal
Port-a-Cul tube
Anaerobic fluid vials
Dry swab (no pledget or sponge)
Methods
Rapid Polymerase Chain Reaction (PCR) using Light Cycler and Fluorescent Resonance Energy Transfer (FRET)
Clinical Utilities
Diagnosis of infections due to Mycoplasmoides pneumoniae
CPT Codes
87581
* The CPT codes provided are based on AMA guidelines and are for informational purposes only. CPT coding
is the sole responsibility of the billing party. Please direct any questions regarding coding to the payer being billed.
